Staple Food Research Articles

Enhanced radiation use efficiency and grain filling rate as the main drivers of grain yield genetic gains in the CIMMYT elite spring wheat yield trial

Common wheat (Triticum aestivum L.) is a major staple food crop, providing a fifth of food calories and proteins to the world’s human population. Despite the impressive growth in global wheat production in recent decades, further increases in grain yield are required to meet future demands. Here we estimated genetic gain and genotype stability for grain yield (GY) and determined the trait associations that contributed uniquely or in combination to increased GY, through a retrospective analysis of top-performing genotypes selected from the elite spring wheat yield trial (ESWYT) evaluated internationally during a 14-year period (2003 to 2016). Fifty-six ESWYT genotypes and four checks were sown under optimally irrigated conditions in three phenotyping trials during three consecutive growing seasons (2018–2019 to 2020–2021) at Norman E. Borlaug Research Station, Ciudad Obregon, Mexico. The mean GY rose from 6.75 (24th ESWYT) to 7.87 t ha−1 (37th ESWYT), representing a cumulative increase of 1.12 t ha−1. The annual genetic gain for GY was estimated at 0.96% (65 kg ha−1 year−1) accompanied by a positive trend in genotype stability over time. The GY progress was mainly associated with increases in biomass (BM), grain filling rate (GFR), total radiation use efficiency (RUE_total), grain weight per spike (GWS), and reduction in days to heading (DTH), which together explained 95.5% of the GY variation. Regression lines over the years showed significant increases of 0.015 kg m−2 year−1 (p < 0.01), 0.074 g m−2 year−1 (p < 0.05), and 0.017 g MJ−1 year−1 (p < 0.001) for BM, GFR, and RUE_total, respectively. Grain weight per spike exhibited a positive but no significant trend (0.014 g year−1, p = 0.07), whereas a negative tendency for DTH was observed (− 0.43 days year−1, p < 0.001). Analysis of the top ten highest-yielding genotypes revealed differential GY-associated trait contributions, demonstrating that improved GY can be attained through different mechanisms and indicating that no single trait criterion is adopted by CIMMYT breeders for developing new superior lines. We conclude that CIMMYT’s Bread Wheat Breeding Program has continued to deliver adapted and more productive wheat genotypes to National partners worldwide, mainly driven by enhancing RUE_total and GFR and that future yield increases could be achieved by intercrossing genetically diverse top performer genotypes.

The Paradox of Alcohol and Food Affordability: Minimal Impact of Leading Beer and Cachaça Brands on Brazilian Household Income Amid Hazardous Drinking Patterns

Alcohol consumption, associated with various cancers, mental disorders, and aggressive behavior, leads to three million deaths globally each year. In Brazil, the alcohol per capita consumption among drinkers aged 15 and over is 41.7 g of pure alcohol/day (~1 L beer/day), which falls into the risky consumption category and exceeds the global average by almost 30%. An effective way to mitigate alcohol-related harm is to increase its retail price. This study assesses the costs of consuming leading brands of beer and sugarcane spirit cachaça (Brazil’s most popular alcoholic beverages) against the expenditure on staple foods. Data on food and alcoholic beverage prices were collected in João Pessoa, Brazil, for 2020 and 2021. The cost per gram of pure alcohol and food were considered to establish consumption patterns of 16.8 g/day (moderate), 41.7 g/day, and 83.4 g/day (heavy), distributed in three scenarios involving the beverages alone or combined (64% beer and 36% cachaça), and a balanced 2000 kcal/day staple diet. The study finds that all heavy consumption scenarios cost less or significantly less (cachaça alone) than a 2000 kcal/day staple diet, highlighting an urgent need for fiscal policies, such as a minimum unit pricing for alcohol, to address public health concerns.

Combined Effect of Biological and Organic Fertilizers on Agrobiochemical Traits of Corn (Zea mays L.) under Wastewater Irrigation

Corn (Zea mays L.) is an important annual grain that is cultivated as a food staple around the world. The current study examined the effect of wastewater and a combination of biological and organic fertilizers on the morphological and phytochemical traits of corn, using a factorial experiment based on a randomized complete block design with three replications. The first factor was biological and organic fertilizers at seven levels, including the control (no fertilization), bacterial biological fertilizers (NPK) along with iron and zinc Barvar biofertilizers, fungal biofertilizers made from Mycorrhiza and Trichoderma, biochar, a combination of bacterial and fungal biofertilizers, and a combination of bacterial and fungal biofertilizers with biochar. The second factor was irrigation at two levels (conventional irrigation and irrigation with wastewater). The traits studied included the morphological yield, phenols, flavonoids, polyphenols, glomalin, cadmium content in plant parts, and translocation factor (TF). The results disclosed that the best treatment in regard to the morphological traits was related to conventional water + biochar + mycorrhiza + Trichoderma + NPK. The highest phenol and flavonoid content were observed when biochar + mycorrhiza + Trichoderma + NPK treatments were used in both water treatments. Also, the wastewater + biochar + mycorrhiza + Trichoderma + NPK treatment demonstrated the highest total glomalin and phenylalanine ammonia-lyase (PAL) activity. The obtained results demonstrate that combined biological and organic fertilizer use on corn plants can effectively alleviate the deleterious effects of cadmium present in wastewater.

Nutrition policy or price stabilisation policy: which policy is more effective for nutrition outcomes?

BackgroundMalnutrition remains a global problem and is increasing with the emergence of the COVID-19 pandemic. In Madagascar, half of the children under five years of age suffer from stunting. However, since 2006, vitamin A supplementation campaigns, deworming and free vaccinations have been implemented within the framework of the Mother and Child Health Week (MCHW) to strengthen the fight against micronutrient deficiencies and reduce mortality. On the other hand, rice, the staple food of the Malagasy population, can provide some of the micronutrients necessary for good nutrition. However, the country’s rice production is still insufficient, and the price has been rising steadily in recent years. This has led the government to resort to the policy of stabilizing rice prices through imported rice in 2017 and 2018. The aim of this paper is therefore to analyse the effects of these policies on the prevalence of malnutrition among children under five years of age in Madagascar. Which policy would be more effective: the nutrition policy or the price stabilisation policy?MethodsData from the Multiple Indicator Cluster Survey conducted by the National Institute of Statistics in 2018 are used, and logistic regressions for the analysis of the effects of nutrition policies on nutrition outcomes are performed. For the effect of price stabilisation policy, panel data on 22 regions of Madagascar from 2016 to 2019 are considered, and a fixed effect model is used.ResultsWe found that the effects of the nutrition policy are not immediately visible. Only participation in the 2016 MCHW contributes to a lower probability of malnutrition occurrence. The odds ratios of the effects of this participation on stunting are 0.69 (p-value: 0.05); on underweight: 0.70 (p-value < 0.01); and on wasting: 0.57 (p-value < 0.01). However, the participation rate remains very low. Then, there is no effect of the rice price stabilization policy on nutrition outcomes (0.00; p-value = 0.11).ConclusionsPrice stabilisation policy is not sufficient to fight against malnutrition, due to a lack of food diversification. These results suggest the need for a policy to ensure nutritional intake and to sensitize the population to participate in the MCHW.Trial registrationNot applicable.

Diet choices determine mercury exposure risks for people living in gold mining regions of Peru

Artisanal and small-scale gold mining (ASGM) is the largest global anthropogenic mercury (Hg) source and is widespread in the Peruvian Amazon. Consuming Hg-laden foods exposes people to this potent neurotoxin. While numerous studies have examined fish Hg content near ASGM, Hg accumulation in other commonly consumed animal-and plant-based foods from terrestrial environments is often overlooked. In this study, we aim to address understudied dietary Hg exposures.To understand Hg exposure from food staples in the Peruvian Amazon, we measured total and methyl Hg in local crops, fish, chicken meat, chicken feathers, and eggs from ASGM-impacted and upstream (reference) communities. Diet surveys were used to estimate probable weekly Hg intake from each food. Fish and chicken stable carbon and nitrogen isotope signatures were analyzed to evaluate trophic magnification.Though few crops exceeded food safety recommendations, rice methyl Hg proportions were high (84%). Trophic level was an expected key predictor of fish Hg content. 81% (17 of 21) of local carnivorous fish exceeded WHO and EPA recommendations. Compared to upstream communities, mining-impacted communities demonstrated elevated total Hg in crops (1.55 (interquartile ranges (IQR): 0.60–3.03) μg kg−1 upstream versus 3.38 (IQR: 1.62–11.58) in mining areas), chicken meats (2.69 (IQR: BDL–9.96) μg kg−1 versus 19.68 (IQR: 6.33–48.1)), and feathers (91.20 (IQR: 39.19–216.13) μg kg−1 versus 329.99 (IQR: 173.22–464.99)). Chicken meats from mining areas exhibited over double the methyl Hg concentrations of those upstream. Methyl Hg fractions in chicken muscle tissue averaged 93%. Egg whites and livers exceeded Hg recommendations most frequently. Proximity to mining, but not trophic position, was a predictor of chicken Hg content.Our results demonstrate that terrestrial and aquatic foods can accumulate Hg from mining activity, introducing additional human Hg exposure routes. However, locally sourced carnivorous fish was the largest contributor to an estimated three-fold exceedance of the provisional tolerable weekly Hg intake.

Rice Brown Planthopper, Nilaparvata lugens (Stål) Feeding Behaviour in Resistant NAGINA22 Rice Mutants

Background: Rice (Oryza sativa L.), is an important staple food and an excellent source of calories in India. Of the different insect pests attacking rice, brown planthopper, BPH, Nilaparvata lugens (Stål) is an important sucking pest causing upto 100% yield losses. Host plant resistance is the most important method in keeping BPH under control and understanding the underlying mechanisms of resistance is necessary to breed the resistant varieties with desirable characters. Methods: Selected EMS mutant lines of Nagina22 (N22) variety, which were previously mass screened under controlled greenhouse conditions were assessed for their antixenosis mechanism to brown planthopper feeding by measuring probing marks and honeydew excretion area by following standard procedures and statistical analysis was done by using Statistix 8.1 software. Result: Both the brown planthopper nymphs and adults probed more in the resistant mutants compared to the susceptible mutants whereas the BPH adults probed more times (24.4) compared to the nymphs (22.2). The recorded honeydew excretion area by the resistant mutants was lower than that of susceptible mutants. In general, the BPH adults fed more and excreted more honeydew (79.5 mm2) compared to the nymphs (59.0 mm2). The damage score has a negative correlation with the probing marks of adults (-0.207) and nymphs (-0.411); and a positive correlation with honeydew excretion of adults (0.547) and nymphs (0.200). In this study, the EMS N22 mutants resistant to BPH, with a greater number of probing marks and less honeydew excretion area can serve as best donors in the breeding programmes to develop brown planthopper resistant varieties.

Nature-based solutions to increase rice yield: An experimental assessment of the role of birds and bats as agricultural pest suppressors in West Africa

Rice is widely consumed as a staple food, being cultivated worldwide. However, in West Africa, production is not enough to satisfy demand. Rice often suffers intensive damage by herbivorous arthropods that affect quality and quantity of the grain. Birds and bats have been shown to suppress arthropod pests, potentially enhancing rice productivity and food security. However, the degree to which these taxa provide nature-based solutions for mitigating pest-induced rice losses is poorly known, especially in West Africa. Here, we used experimental exclosures to investigate whether birds and bats reduce plant damage and boost rice yield by suppressing arthropod abundance. In a rural area in northern Guinea-Bissau, we established 14 sets of paired control and experimental exclosures parcels, precluding access of birds and bats to rice plants. We then quantified how the absence of birds and bats influenced arthropod communities, plant damage, and rice yield over a full rice production cycle (six months). Arthropod numbers in exclosures (10.1 ± 9.1 ind./plot) were nearly double those in control plots (5.8 ± 3.0 ind./plot), a result mostly due to a lower spider abundance in the controls. The percentage of leaf and grain damage showed no difference between exclosure and control. Using Structural Equation Models, we uncovered that the exclusion of birds and bats boosted arthropod abundance but had only marginal effects on rice damage and no detectable effect on yield. The exclusion of flying vertebrates led to a marked increase in spider abundance, suggesting an effect of mesopredator release, which in turn likely helped maintaining pest abundance low and potentially contributing to the small overall effect on rice damage and yield. Enhancing the abundance of birds and bats is an interesting option to suppress agricultural pests, but our results highlight the need for a better understanding of ecological interactions in agricultural landscapes in West Africa. We stress the need for more research to inform evidence-based policies using nature-based solutions that foster the natural consumption of pests by vertebrates, as a means to improve food security.

Identification of cysteine-rich receptor-like kinase gene family in potato: revealed StCRLK9 in response to heat, salt and drought stresses

The investigation into cysteine-rich receptor-like kinases (CRLKs) holds pivotal significance as these conserved, upstream signalling molecules intricately regulate fundamental biological processes such as plant growth, development and stress adaptation. This study undertakes a comprehensive characterisation of CRLKs in Solanum tuberosum (potato), a staple food crop of immense economic importance. Employing comparative genomics and evolutionary analyses, we identified 10 distinct CRLK genes in potato. Further categorisation into three major groups based on sequence similarity was performed. Each CRLK member in potato was systematically named according to its chromosomal position. Multiple sequence alignment and phylogenetic analyses unveiled conserved gene structures and motifs within the same groups. The genomic distribution of CRLKs was observed across Chromosomes 2–5, 8 and 12. Gene duplication analysis highlighted a noteworthy trend, with most gene pairs exhibiting a Ka/Ks ratio greater than one, indicating positive selection of StCRLKs in potato. Salt and drought stresses significantly impacted peroxidase and catalase activities in potato seedlings. The presence of diverse cis-regulatory elements, including hormone-responsive elements, underscored their involvement in myriad biotic and abiotic stress responses. Interestingly, interactions between the phytohormone auxin and CRLK proteins unveiled a potential auxin-mediated regulatory mechanism. A holistic approach combining transcriptomics and quantitative PCR validation identified StCRLK9 as a potential candidate involved in plant response to heat, salt and drought stresses. This study lays a robust foundation for future research on the functional roles of the CRLK gene family in potatoes, offering valuable insights into their diverse regulatory mechanisms and potential applications in stress management.

Phytoparasitic Nematodes of Musa spp. with Emphasis on Sources of Genetic Resistance: A Systematic Review

Bananas are a staple food that considerably contributes to both food security and income generation, especially in countries of Africa, Asia, and Central and South America. The banana plant (Musa spp.) is affected by various pathogens, of main concern being the plant-parasitic nematodes associated with the rhizosphere, the most important of which are Radopholus similis (burrowing nematode), Helicotylenchus sp. (spiral nematode), Pratylenchus sp. (root lesion nematode), and Meloidogyne sp. (gall nematode). Infected plants reduce their ability to absorb water and nutrients, which can lead to delayed flowering, fewer bunches, and lower fruit mass. Obtaining nematode-resistant banana cultivars through genetic improvement is an effective and sustainable option compared with chemical control with nematicides. Here, we provide the first systematic review of existing banana sources of resistance to nematodes to aid the management and control of nematodes in banana and plantain crops. Articles selected from different databases were evaluated, and searches were conducted using pre-established inclusion and exclusion criteria. We found 69 studies dealing with genetic improvement for nematode resistance in banana cultivation. Our findings revealed that sources of resistance are currently under investigation to combat the diseases caused by different nematode species in banana plants.

The nutritional benefits of maize-soybean rotational systems in the North-Western Free State, South Africa

BackgroundMalnutrition is one of the major health concerns, particularly in the developing and under-developed world. In South Africa, maize is produced as a staple food and is the primary food for most of the country’s population. The North-Western Free State which forms part of the Nala municipality in the Lejweleputswa district of South Africa is a main producer of the country’s maize. However, the area is known for its sandy soil which contains little organic material, silt and clay. Maize in this area is normally grown in monoculture but with a focus on sustainable agriculture has recently incorporated soybean. As a means of fighting malnutrition, the objective of this study was to determine the influence that soybean incorporation as a rotation crop has on the nutritional value of maize.MethodsA trial was conducted on the farm Christinasrus in the North-Western Free State to compare the nutritional value of monoculture maize and maize in rotation with soybean over three consecutive seasons. Maize kernel samples were taken each season and its nutritional properties analyzed. Subsequent data were further analyzed using statistical analysis.ResultsResults showed that there was a seasonal effect on all nutritional properties with a general decrease in nutritional values in wetter years. Cropping systems had an effect on fibre content, with increased values observed in maize after soybean. In addition, there was a significant interaction between season and cropping systems on the total digestible nutritional value, with maize after soybean being more nutritious in wetter seasons.ConclusionResults suggest that maize in cropping systems with soybean has potential to be more nutritious while the soybean in the cropping system can act as a protein-rich companion, providing a more balanced diet for human consumption, thereby fighting malnutrition.

Bayesian network analysis of factors influencing type 2 diabetes, coronary heart disease, and their comorbidities

ObjectiveBayesian network (BN) models were developed to explore the specific relationships between influencing factors and type 2 diabetes mellitus (T2DM), coronary heart disease (CAD), and their comorbidities. The aim was to predict disease occurrence and diagnose etiology using these models, thereby informing the development of effective prevention and control strategies for T2DM, CAD, and their comorbidities.MethodEmploying a case-control design, the study compared individuals with T2DM, CAD, and their comorbidities (case group) with healthy counterparts (control group). Univariate and multivariate Logistic regression analyses were conducted to identify disease-influencing factors. The BN structure was learned using the Tabu search algorithm, with parameter estimation achieved through maximum likelihood estimation. The predictive performance of the BN model was assessed using the confusion matrix, and Netica software was utilized for visual prediction and diagnosis.ResultThe study involved 3,824 participants, including 1,175 controls, 1,163 T2DM cases, 982 CAD cases, and 504 comorbidity cases. The BN model unveiled factors directly and indirectly impacting T2DM, such as age, region, education level, and family history (FH). Variables like exercise, LDL-C, TC, fruit, and sweet food intake exhibited direct effects, while smoking, alcohol consumption, occupation, heart rate, HDL-C, meat, and staple food intake had indirect effects. Similarly, for CAD, factors with direct and indirect effects included age, smoking, SBP, exercise, meat, and fruit intake, while sleeping time and heart rate showed direct effects. Regarding T2DM and CAD comorbidities, age, FBG, SBP, fruit, and sweet intake demonstrated both direct and indirect effects, whereas exercise and HDL-C exhibited direct effects, and region, education level, DBP, and TC showed indirect effects.ConclusionThe BN model constructed using the Tabu search algorithm showcased robust predictive performance, reliability, and applicability in forecasting disease probabilities for T2DM, CAD, and their comorbidities. These findings offer valuable insights for enhancing prevention and control strategies and exploring the application of BN in predicting and diagnosing chronic diseases.

Unlocking Nutritional Potential: Multi-OMICS Strategies for Enhancing Millet Nutritional Traits

Millet grains have long been recognized for their nutritional significance, serving as staple foods for millions around the world. However, unlocking their full nutritional potential remains a crucial challenge. In this study, we explore the application of multi-OMICS (genomics, transcriptomics, proteomics, metabolomics, and phenomics) strategies to enhance the nutritional traits of millet grains. By integrating comprehensive molecular datasets, we aim to elucidate the genetic and biochemical pathways governing key nutritional attributes such as protein content, amino acid composition, micronutrient density, and antioxidant capacity in millets. Through targeted breeding and biotechnological interventions informed by multi-OMICS analyses, we seek to develop improved millet varieties with enhanced nutritional profiles and agronomic performance. This interdisciplinary approach holds great promise for addressing malnutrition and promoting food security, particularly in regions where millets are dietary staples. By harnessing the power of multi-OMICS technologies, we aim to unleash the full nutritional potential of millets, contributing to the development of sustainable and resilient food systems for future generations.

Development of Analog Rice Made from Cassava and Banana with the Addition of Katuk Leaf (Sauropus androgynous L. Merr.) and Soy Lecithin for Lactating Women

The development of analog rice, apart from being an effort to diversify food, also has the potential to be developed as a functional food to fulfill the nutrients needed by a community. Katuk leaf is known for its ability to accelerate the breast milk production of lactating women, which is inseparable from sterol in terms of bioactive content. This study aimed to determine the best formulation of analog rice made from cassava flour, banana flour, Katuk leaf powder, and soy lecithin that was sensorily acceptable, in a shape resembling rice, and able to fulfill the nutritional needs of lactating women. Analog rice was produced using an extruder machine before the physical and sensory properties analyses were carried out, followed by the chemical properties analysis. Formulation C (80% cassava flour, 20% banana flour, 3% Katuk leaf powder, and 0.5% soy lecithin) was obtained as the best or most preferred formulation based on sensory analysis. The resulting grain was oval–round in shape and had a green-brownish color, fluffy texture, and distinct aroma and taste derived from the raw material used. Therefore, this research is expected to support the development of analog rice for providing the main staple food to fulfill lactating women’s nutrition.

Assessment of Biochemical Parameters and Yield Performance in Rice under Different Crop Establishment Methods

Rice (Oryza sativa L.), a staple food for half of the global population, is cultivated using various methods, with transplanting being conventional in many Asian countries. However, challenges such as high-water consumption, labour intensiveness, and environmental degradation have prompted the exploration of alternative methods. In this study, we assessed the impact of various crop establishment methods (CE) i.e. conventional puddled transplanting, direct drill seeding on flatbed (DSR), and direct seeding on raised beds (FIRB) on five stress-tolerant rice varieties (V) i.e. DRR 42, DRR 44, Sukha Dhan 5, Sukha Dhan 6 and Sarjoo 52 by analysing biochemical parameters i.e. total sugar content, starch content, MDA content, SOD content and yield outcomes. Our findings reveal significant variations in biochemical parameters and yield across different CE and V combinations. Notably, FIRB consistently outperformed other CEs, indicating its potential for enhancing stress tolerance and yield. Similarly, DRR 44 exhibited superior performance across most growth stages. Our study highlights the potential advantages of FIRB method in mitigating water wastage and addressing the limitations of conventional transplanting practices.

Scales of plant stewardship in the precontact Pacific Northwest, USA

Numerous oral histories and substantial ethnographic evidence illustrate how plant species, communities, and even landscapes were extensively managed and cared for by ancestral communities in the Pacific Northwest. Camas ( Camassia spp.) is one such cultural keystone plant, common from the Pacific Ocean to the Rocky Mountains, with numerous records describing its role as a staple food for many Northwest peoples. Supporting deep time archeological evidence for such management or stewardship practices, however, has remained elusive. In this paper we analyze archived collections of archeological camas bulbs from 11 sites across the Willamette Valley, Oregon to demonstrate people began preparing camas within earth ovens by approximately 8000 calendar years before present and deliberately harvesting sexually mature camas plants circa 3500 calendar years before present. We compare these findings with climatological, palynological, and fire history reconstructions to discuss stewardship strategies for camas and associated plant communities through time at the population, community, and landscape levels. These findings confirm and expand upon Indigenous knowledges as well as offer time-tested methods for cultural keystone conservationists seeking to revitalize traditional plant stewardship practices throughout this region and beyond. This “camas case study” also offers another example of a human-plant symbiotic relationship, expanding our knowledge of plant food pathways, processes, and mutualisms.

OsCAMTA3 Negatively Regulates Disease Resistance to Magnaporthe oryzae by Associating with OsCAMTAPL in Rice.

Rice (Oryza sativa) is one of the most important staple foods worldwide. However, rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, seriously affects the yield and quality of rice. Calmodulin-binding transcriptional activators (CAMTAs) play vital roles in the response to biotic stresses. In this study, we showed that OsCAMTA3 and CAMTA PROTEIN LIKE (OsCAMTAPL), an OsCAMTA3 homolog that lacks the DNA-binding domain, functioned together in negatively regulating disease resistance in rice. OsCAMTA3 associated with OsCAMTAPL. The oscamta3 and oscamtapl mutants showed enhanced resistance compared to wild-type plants, and oscamta3/pl double mutants showed more robust resistance to M. oryzae than oscamta3 or oscamtapl. An RNA-Seq analysis revealed that 59 and 73 genes, respectively, were differentially expressed in wild-type plants and oscamta3 before and after inoculation with M. oryzae, including OsALDH2B1, an acetaldehyde dehydrogenase that negatively regulates plant immunity. OsCAMTA3 could directly bind to the promoter of OsALDH2B1, and OsALDH2B1 expression was decreased in oscamta3, oscamtapl, and oscamta3/pl mutants. In conclusion, OsCAMTA3 associates with OsCAMTAPL to regulate disease resistance by binding and activating the expression of OsALDH2B1 in rice, which reveals a strategy by which rice controls rice blast disease and provides important genes for resistance breeding holding a certain positive impact on ensuring food security.

Agroecosystem-based analysis of household dietary diversity and its linkage with agricultural production diversification in the Upper Blue Nile Basin, Ethiopia: Multilevel analysis approach

Even though the UN's Sustainable Development Goals emphasize improving dietary diversity and overall nutritional status globally by promoting sustainable agriculture, inadequately diversified dietary intake remains a public health problem in many low-income countries, including in Sub-Saharan Africa. The main objective of this study was to gather relevant information to gain an improved understanding of household dietary diversity and explore the role that production diversification could play within household diets and nutrition so that appropriate interventions could be implemented with the goal of enhancing nutrition-sensitive agriculture while also considering the varied features across the agroecosystems. Agroecosystem-based cross-sectional study design was employed. Data were collected predominantly through semi-structured questionnaires and analyzed by descriptive and inferential statistics, and a multilevel mixed-effect model. Results showed that the usual dietary intake of households has a monotonous pattern dominated by starchy staple foods, and consumption of fruits, vegetables, and animal-sourced foods was very limited. One out of five households had inadequate dietary diversity but varied significantly across agroecosystems. About 26% of the heterogeneity in dietary diversity was attributed due to agroecosystem level factors. In the fixed-effect part of the model, production diversification, wealth status, number of meals per day, market access, and annual mean rainfall were significantly associated with household dietary diversity status. The findings of our research contribute to give insights on the linkage between dietary diversity and production diversification among subsistence rural farmers across contrasting agroecosystem zones. Therefore, understanding agroecosystem variations and local context in the rural subsistence farming setup is a key point in designing nutrition-sensitive agriculture.

Comparative transcriptomics of seed nourishing tissues: uncovering conserved and divergent pathways in seed plants.

The evolutionary and ecological success of spermatophytes is intrinsically linked to the seed habit, which provides a protective environment for the initial development of the new generation. This environment includes an ephemeral nourishing tissue that supports embryo growth. In gymnosperms this tissue originates from the asexual proliferation of the maternal megagametophyte, while in angiosperms it is a product of fertilization, and is called the endosperm. The emergence of these nourishing tissues is of profound evolutionary value, and they are also food staples for most of the world's population. Here, using Orthofinder to infer orthologue genes among newly generated and previously published datasets, we provide a comparative transcriptomic analysis of seed nourishing tissues from species of several angiosperm clades, including those of early diverging lineages, as well as of one gymnosperm. Our results show that, although the structure and composition of seed nourishing tissues has seen significant divergence along evolution, there are signatures that are conserved throughout the phylogeny. Conversely, we identified processes that are specific to species within the clades studied, and thus illustrate their functional divergence. With this, we aimed to provide a foundation for future studies on the evolutionary history of seed nourishing structures, as well as a resource for gene discovery in future functional studies.

Enhancing Protein and Vitamin A Intake Through the Addition of Bambara Groundnut and Ripe Plantain to Maize Porridge

ABSTRACTProtein deficiency, especially among children, is prevalent in most developing countries. One sustainable strategy proposed to tackle this deficiency is by incorporating locally available but underutilised staple foods that are rich in proteins such as Bambara groundnut into pre‐existing and popular diets such as porridge prepared from roasted maize flour. This strategy can also be used to tackle vitamin A deficiency which is common among children and pregnant women through the incorporation of ripe plantain—a vitamin A rich staple. In this study, the quality of composite flours prepared from roasted maize, Bambara groundnut and ripe plantain and the acceptability of porridge made thereof was investigated. Increasing the levels of Bambara groundnut and ripe plantain increased the protein and β‐carotene levels, respectively, without affecting the physicochemical quality. Increasing the content of ripe plantain increased the iron content; however, higher levels of the Bambara groundnut increased the tannin content. Flash profiling showed that flour composition influenced the attributes of the porridges; however, hedonic sensory scores showed no differences in acceptability with respect to the aroma, appearance, texture and taste.

CRISPR/Cas9-generated mutations in a sugar transporter gene reduce cassava susceptibility to bacterial blight.

Bacteria from the genus Xanthomonas are prolific phytopathogens that elicit disease in over 400 plant species. Xanthomonads carry a repertoire of specialized proteins called transcription activator-like (TAL) effectors that promote disease and pathogen virulence by inducing expression of host susceptibility (S) genes. Xanthomonas phaseoli pv. manihotis (Xpm) causes bacterial blight on the staple food crop cassava (Manihot esculenta Crantz). The Xpm effector TAL20 induces ectopic expression of the S gene Manihot esculenta Sugars Will Eventually be Exported Transporter 10a (MeSWEET10a), which encodes a sugar transporter that contributes to cassava bacterial blight susceptibility. We used CRISPR/Cas9 to generate multiple cassava lines with edits to the MeSWEET10a TAL20 effector binding site and/or coding sequence. In several of the regenerated lines, MeSWEET10a expression was no longer induced by Xpm, and in these cases, we observed reduced cassava bacterial blight (CBB) disease symptoms post Xpm infection. Because MeSWEET10a is expressed in cassava flowers, we further characterized the reproductive capability of the MeSWEET10a promoter and coding sequence mutants. Lines were crossed to themselves and to wild-type plants. The results indicated that expression of MeSWEET10a in female, but not male, flowers, is critical to produce viable F1 seed. In the case of promoter mutations that left the coding sequence intact, viable F1 progeny were recovered. Taken together, these results demonstrate that blocking MeSWEET10a induction is a viable strategy for decreasing cassava susceptibility to CBB and that ideal lines will contain promoter mutations that block TAL effector binding while leaving endogenous expression of MeSWEET10a unaltered.